FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAACRAAPEI-205_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAACRAAPEI-205_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35021410
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGTTCCTGGTGCTCCTTGGAGCTGCCTTTGCTCTGGATGATGAC1301960.37176115981623814No Hit
CAAGAATCGGTTTTAGTTGTTTCTCATGGTGTCAGCGATCCACTGGCTGA564580.16120995699487828No Hit
GTTCAATCAGCAGTCATGAGGTCTTTGGTGTTCCTGGTGCTCCTTGGAGC444820.12701373245680284No Hit
CAAACATGCTGAGGATCCTGTTGTTGAGTGTGCTGGCCGCTTTGGCCCTG415050.11851321805718275No Hit
CTTGGAGCTGCCTTTGCTCTGGATGATGACAAGATTGTTGGTGGATATGA370060.10566679068604035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAAT79200.026.8585821
GAATCGG98100.026.3255334
AATCGGT103700.024.9675395
AGAATCG103900.024.9403653
ATCGGTT119950.021.7318216
TTCAATC105550.021.6433412
CGGTTTT130550.019.8662078
TCGGTTT137800.019.060457
ATCGGAT16150.016.8899046
GGGATTG43300.016.76482811
TGCCGTA154100.016.70253431
CAAGAAT178400.016.3766231
CGGATAG15750.016.061848
ACAATCG20000.015.9481783
CCGTAAA163000.015.85828433
CAATCAG145750.015.83233454
AATCGGA18450.015.618995
TCGTGTA49400.015.5928772
TCAATCA162200.015.5284363
AAGAATC190150.015.4746292
Produced by FastQC (version 0.11.5)